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Liver myeloperoxidase activity To determine liver myeloperoxidase (MPO) activity, liver tissue was homogenized in 1 ml of cold hexdecyltrimethylammonium bromide buffer, sonicated on ice for 10 s and centrifuged at 14,000xg for 30 min at 4 C
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Indeed, exposure of tumor cells to elevated, yet physiologically relevant, pressure, using a pressurized cultured system that maintains a constant pH, has been found to increase or decrease cell proliferation, depending on the cancer cell type [51]
Analytical identification and characterisation
For antigen retrieval, slides were immersed in citrate buffer pH 6.0 and heated in a Decloaking Chamber 30 seconds at 125 C, followed by 10 seconds at 90 C
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