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(B) Cells given 0.4 mM palmitic acid (PA) were treated with either vehicle or 50 to 100 nM Ex-4 for 24 hours

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GLP-1R binding to VAPB requires GLP-1R internalisation and is differentially modulated by GLP-1RAs with varying GLP-1R internalisation propensities To elucidate whether active GLP-1R requires its prior internalisation to engage VAPB, we co-transfected INS-1 832/3 SNAP/FLAG-hGLP-1R cells with dominant negative (K44A) mutants of dynamin 1 and 2 to block GLP-1R endocytosis triggered by exendin-4 (Supplementary Fig