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After washing with PBS-T five times, secondary antibody-incubated membranes were imaged with the ChemiDocMP Imaging System (BIO-RAD Laboratories) directly with the IRDye800CW channel for visualisation of vinculin bands, or with the addition of western blotting detection reagent (SuperSignal West Dura Extended Duration Substrate, Thermo Fisher Scientific, #34075) for the HRP conjugated system with chemiluminescence channel

JBMR Plus (2019) 3(10):e10209

Syringe type: U-100 insulin (100 units = 1 mL), U-50 insulin (50 units = 0.5 mL), or standard mL markings

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2021-I2M-1-028)

By reducing cytokine activity, Bepecin helps decrease pain and swelling, improving mobility and comfort