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the samples were then incubated at 37 C overnight on a shaker

This modification shifts the charge of lysine from +1 to 1, thereby altering the protein structure and interfering with electrostatic interactions ( 94 )

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Even modest reductions in micronutrient availability may therefore influence catalytic velocity across interconnected metabolic pathways, potentially amplifying bioenergetic and redox constraints under chronic flux limitation [112, 113]

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In this study, we find that uridine supplementation, both in vivo and in vitro, significantly enhances oocyte maturation, fertilization potential, and early embryonic development in aged mice, ultimately improving fertility