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For the optogenetic experiments targeting the PVN Glu DMV pathway, we injected AAV-DIO-ChR2-mCherry or AAV-DIO-mCherry control virus into the PVN of VgluT2-Cre mice

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GHK-Cu (Glycyl-L-Histidyl-L-Lysine Copper)is a well-studied copper-binding peptide that has generated substantial interest within the fields of regenerative biology, molecular signaling, and tissue repair research

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