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2 Identification of the other hormone, GLP-1, was a more complex task because of the difficulty of differentiating it immunologically from other bioactive products and inactive degradation fragments of its precursor, proglucagon

The slides were washed in water/PBS and then blocked using 5% goat serum in PBS for 1 h at room temperature prior to adding primary antibodies diluted in the blocking buffer

Samples were spun down at 1,000 g for 5 min at 4 C and the supernatant containing tag-free laccases was collected

Some may experience temporary nasal irritation after use

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When unfolded, the mask is about this size