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The AML molecular landscape was explored at multiple omic layers, including WGS (173 tumors, 140 normals), WES (173 tumors, 161 normals), RNA-seq (172 tumors), proteomics including PTMs (162 tumors), metabolomics (91 tumors) and lipidomics (96 tumors) (Supplementary Table 1)

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Interestingly, the transcription factor encoding the early B cell factor 3 ( Ebf3 ) gene was found to be one of the distinctive markers for the low-TH expressing subgroup among the Pitx3 isolated mDA neurons

Cells were then incubated at 37 C for the indicated time points in a mixture containing 1x Hanks balanced salt solution (HBSS, Gibco) or DMEM, each supplemented with 1 mM MgCl 2 , and 125 U Benzonase nuclease (Sigma/Pierce)