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These obtained results support conclusion to a role of SEMA on stroma enrichment decrease paralleled to T lymphocyte infiltration increase

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coli (New England Biolabs), recovered in 20 ml 2YT medium supplemented with 25 mM glucose for 10 min, and transferred into 200 ml terrific broth supplemented with 50 g ml 1 carbenicillin for overnight recovery at 37 C in a 220-rpm shaking incubator