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doi: 10.1200/JCO.2004.10.105

Organic compounds as contaminants of the Elbe river and its tributaries

Ohnishi, K

For metabolic analysis of cultured cancer cells and tumours, frozen tumour tissue was homogenized in 80% methanol containing 20 mM NEM using a Precellys cold tissue homogenizer (Bertin) at a ratio of 20 mg tissue to 1 ml solvent