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The sections were then incubated with secondary antibodies, including Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor Plus 488 (Invitrogen, A32790, 1:500) and Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody Alexa Fluor 594 (Invitrogen, A-11001, 1:500)

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These patterns rarely have a single cause, which is why we assess rather than assume

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