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Neutralising Pns11-sperm binding To detect the direct interaction between Pns11 and Pelo on sperms, the mature live sperms excised from nonviruliferous males were pre-incubated with pre-immune or Pelo antibody (0.5 g/L) for 30 min and then incubated with purified Pns11 proteins (1.0 g/L) for 1 h

The resistance of encapsulated AuNCs to photobleach in our study agrees with an increasing body of literature supporting the outstanding photostability of gold nanoclusters 16,52,53,54

10.1210/endo.140.8.6886 62 Raffalli-MathieuF.OrreC.StridsbergM.Hansson EdalatM.MannervikB

Supernatants may be isolated by centrifugation at appropriate speed and duration (e.g., 13,000 rpm for 1 min, 500 rpm for 5 min, etc.)

10.1016/j.scitotenv.2023.164619 495 YangQ.QinB.HouW.QinH.YinF

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