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Indeed, the initial purities of her synthetic materials were so high that a single pass through a preparative HPLC column was sufficient to isolate them for further application [31]

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4-OH-CPA is the major circulating metabolite and is in equilibrium with aldophosphamide, which subsequently breaks down into phosphoramide mustard, the active cytotoxic compound, and acrolein, a toxic byproduct responsible for bladder toxicity