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cDNA was prepared from total RNA and used for gene expression by real-time quantitative RTPCR (qPCR) as described [53] using TaqMan Gene Expression Assays (ThermoFischer Scientific) specific for human GCLC (Hs00155249_m1), OCT4 (Hs00999632_g1), and ACTB (Hs01060665_g1)

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Tosukhowong et al

All three peptides show promising results in preclinical and early clinical studies, but further research is needed to confirm their long-term safety and efficacy

O armazenamento e o manuseio devem seguir os protocolos laboratoriais especficos para cada peptdeo