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Antioxidant enzyme activities A crude enzyme solution was isolated from leaves by grinding 0.3 g of leaf tissue to powder with liquid nitrogen and homogenizing in 3 mL of cold phosphate-buffered saline (PBS) (50 mM, pH 7.8) containing 1% polyvinylpyrrolidone (PVPP) and 0.2 mM ethylenediaminetetraacetic acid (EDTA)

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