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The membranes were blocked with 5% skim milk in TBS/T (20 mM TrisHCl, pH 7.6, 137 mM NaCl and 0.05% Tween 20) and incubated with an anti-pAkt Ser 473 antibody (#9271

Some users also add SS-31 for mitochondrial support or TB-500 for enhanced tissue repair

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And, what happens if you take too much

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This was associated with substantive mobility of the RAMP2 linker and TM helix, which had weaker interaction with the CTR TM bundle compared to RAMP1 and RAMP3 in AMY 1 R and AMY 3 R complexes