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Targeted methods using multiple reaction monitoring (MRM) on a triple quadrupole platform were also developed by quantitatively labeling all available cysteine residues with a labeling reagent yielding isomerically-modified peptides following enzymatic digestion
In contrast, the normal prostate epithelial cell line PNT2 exhibited reduced EV protein cargo following identical LMF exposure, with fewer associated functional enrichment pathways and minimal alterations in miRNA expression or invasion
The review stresses that while promising, large RCTs are missing
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