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Cartilage-specific ablation of XBP1 signaling in mouse results in a chondrodysplasia characterized by reduced chondrocyte proliferation and delayed cartilage maturation and mineralization
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ATPase/GTPase Activity Assay Kit (#MAK113) was purchased from Merck (Darmstadt, Germany)
For data calculation background fluorescence was subtracted before intensiometric TS fluorescence signals were divided by mCherry signals to allow for a ratiometric, probe expression-independent readout
These cells turn out to produce a number of IGFBPs under certain circumstances and it is known that this protein drastically lowers the impact of IGF-1 at its other receptors
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