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For experiments involving caspase-3/7 staining, CellEvent Caspase-3/7 Green Detection Reagent (Thermo Scientific C10723) and CPP-CAND were added to live cells 24 h before imaging using an Eclipse Ti-U inverted microscope (NIKON)

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The pen needle is typically 29-31 gauge and designed for subcutaneous delivery across all body types

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This mimetic action helps to: Stimulate Insulin Release: After a meal, Ozempic encourages the pancreas to release more insulin, which is essential for metabolizing sugar and preventing high blood sugar levels