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The cell suspensions were washed twice with ice-cold PBS before staining using an Annexin V/PI staining kit (Alexa Fluor 488 annexin V/Dead Cell Apoptosis Kit)

These cells release their own mediators, causing prolonged inflammation that can last days

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For lupus patients, this connection carries particular significance

Following a 1-week acclimatization period in the laboratory, the mice were subjected to a hair removal procedure