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BtaGSTA1-1, an alpha class GST identified in tight association with bovine steroidogenically active cells and shown to be hormonally regulated by gonadotropins in the bovine ovarian follicles, appeared to be a likely isomerase ( Based on mutational studies of human GSTs the five H-site residues in positions 10, 12, 111, 208, and 216 were judged to be of particular importance for the ketosteroid isomerase activity (Table 1)