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Individuals with severe MTHFR deficiency are characterised by highly impaired production of 5-MTHF

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Tumours were cut into small pieces (15 mm), put into the enzyme mix in RPMI and dissociated using gentleMACS Octo Dissociator with Heaters (Miltenyi) with the appropriate gentleMACS program (37C_m_TDK)

However, this had a pitfall in that the blood supply for the L flap comes from the alveolus, thus cutting the modified Millards L flap so that the blood supply would come from the lateral nasal wall