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For the cytoplasm and muscle, the sections were then stained in Biebrich scarlet-acid fuchsin solution for 1015 min, again washed with distilled water and treated with phosphomolybdic-phosphotungstic acid solution for 1015 min, at this step, and for collagen staining, slides were transferred to aniline blue solution for 510 min and rinsed with water and 1% acetic acid

Although the exact causes of Parkinsons disease are not fully understood, there is evidence that oxidative stress plays an important role in the pathogenesis of this disease

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J Evol Biochem Physiol 39(4):433438

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